PLPA
652/BIOPL 652 Molecular Plant Pathology
4
February 2002 S. Lazarowitz
A. Virus movement proteins coordinate viral
genome replication with its directed movement within and between cells
1. Models for virus movement: overview of TMV,
SqLCV, ‘tubule’ viruses, PVX (Lazarowitz
and Beachy, 1999).
B. TMV: a model for cytoskeletal trafficking,
and plasmodesmal targeting and gating.
1. 30 kD protein as an RNA binding protein (Citovsky et al., 1990; Citovsky et al.,
1992).
2. 30 kD protein (and other MPs) effects size
exclusion limits of PD (Ding et al.,
1992; Fujiwara et al., 1993; Oparka et al., 1997; Waigmann et al., 1994; Wolf
et al., 1989).
a. Microinjection
vs. in vivo expression of GFP-MP alone
and during infection: what each can and
cannot tell you (Oparka et al., 1997; Oparka et al., 1999).
b. Proteins
as large as 50 kD can move
through PD in sink leaves! (Oparka et al., 1999).
3. Associations with actin filaments
and microtubules (Heinlein et al.,
1995; Heinlein et al., 1998; McLean et al., 1995; Reichel and Beachy, 1998).
4. The 30 kD MP interacts with a cell wall
pectin methylesterase (Chen et al., 2000).
C. SqLCV: a model for nuclear shuttling,
directional movement, and biogenesis of cell wall channels.
1. NSP is a ssDNA binding protein (Pascal et al., 1994).
2. Subcellular localization of NSP and MPB:
nuclear and ER targeting, tubules as potential analog of the PD desmotubule (Pascal et al., 1994; Ward et al., 1997).
3. Cooperative interaction of MPs and nuclear
shuttling shown in living plant cells (Sanderfoot
et al., 1996; Sanderfoot and Lazarowitz, 1995).
4. Identification of a plant nuclear export
signal (Ward and Lazarowitz, 1999).
D. PVX: insights into systemic movement.
1. Virus
particles move cell-to-cell: requirements for CP and ‘triple gene block’ MPs (Angell and Baulcombe, 1995; Angell et
al., 1996; Hefferon et al., 1997; Kalinina et al., 1996; Oparka et al., 1996;
Santa Cruz et al., 1996; Santa Cruz et al., 1998).
2. CP
and smaller two TGB MPs (12K, 8K) interact and modulate function of the largest
TGB MP (25K) (Yang et al., 2000).
2. CP is required for systemic movement (Santa Cruz et al., 1998).
3. Exiting phloem companion cells and entering
sieve elements: disassembling and
reassembling (Santa Cruz et al.,
1998)?
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